You MUST register for ALL of the following:

  • WHEN: January 25th, 1-4 pm
  • WHERE: Falk Library, Classroom 2
  • WHO: Ansuman Chattopadhyay, PhD
  • Massively parallel RNA sequencing, commonly known as RNA-seq, provides a more detailed picture of transcriptomes compared to microarray profiling. As Next-Generation Sequencing technologies are becoming faster and less expensive, RNA-seq is now the primary assay of choice for researchers investigating the abundance and diversity of RNA transcripts. Analysis of the large-scale data sets generated by a typical RNA-seq experiment is challenging as it demands access to powerful computers and researcher training to run sophisticated bioinformatics software packages. This workshop will teach how to analyze sample RNA-seq data using CLCbio Genomics Workbench software and the CLCbio Genomics Server installed at the Pitt FRANK Cluster.

    Participants will gain hands-on experience to:

    • Download biological sequence data from NCBI Sequence Read Archive (SRA)/European Nucleotide Archive (ENA)
    • Align reads to the human reference genome
    • Estimate expression of known genes and transcripts
    • Perform differential expression analysis at the gene and transcript isoform level

    Please contact the HSLS Molecular Biology Information Service with any questions.