Registrations for this workshop have reached capacity. If you show up without having registered AND without having received a confirmation email, you are likely to be turned away due to lack of space. However, we are very likely to offer it again in December or January. Thanks to all of you for your interest!

  • WHEN: November 18th, 1-3pm
  • WHERE: Falk Library, Classroom 2
  • WHO: Ansuman Chattopadhyay, PhD
  • Massively parallel RNA sequencing, commonly known as RNA-seq, provides a more detailed picture of transcriptomes compared to microarray profiling. As Next-Generation Sequencing technologies are becoming faster and less expensive, RNA-seq is now the primary assay of choice for researchers investigating the abundance and diversity of RNA transcripts. Analysis of the large-scale data sets generated by a typical RNA-seq experiment is challenging as it demands access to powerful computers and researcher training to run sophisticated bioinformatics software packages. This workshop will teach how to analyze sample RNA-seq data using CLCbio Genomics Workbench software and the CLCbio Genomics Server installed at the Pitt FRANK Cluster. The results will be further analyzed using NextBio.

    Participants will gain hands-on experience to:

    • Download biological sequence data from NCBI Sequence Read Archive (SRA)/European Nucleotide Archive (ENA)
    • Align reads to the human reference genome
    • Estimate expression of known genes and transcripts
    • Perform differential expression analysis at the gene and transcript isoform level

    Please contact the HSLS Molecular Biology Information Service with any questions.