Register for RNA-Seq Data Analysis using CLC Genomics Workbench
June 22, 11 a.m.-1 p.m., virtual
This is a flipped class; links to PowerPoint slides, lecture videos, and practice exercises that you can view on your own schedule are available upon registration. During this virtual hands-on session, you will learn how to solve the exercise problems.
The workshop lecture video will walk you through the steps of a bulk RNA-seq data analysis process using the HSLS-licensed CLC Genomics Workbench.
Participants will learn how to
- access the CLCbio Genomics Server hosted on the HTC Cluster by Pitt CRC
- import RNA-Seq FASTQ reads from a GEO dataset
- assess the quality of RNA-Seq data
- align reads to a reference genome
- estimate known gene and transcript expression
- perform differential expression analysis
- visualize data by generating PCA and heatmaps
Target Audience:
Experimental biologists seeking to analyze bulk RNA-Seq data generated through experiments or retrieved from a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user interface, so neither programming experience nor familiarity with the command-line interface is required.