This information is over 2 years old. Information was current at time of publication.{"id":11099,"date":"2018-06-20T15:05:18","date_gmt":"2018-06-20T19:05:18","guid":{"rendered":"http:\/\/info.hsls.pitt.edu\/updatereport\/?p=11099"},"modified":"2018-07-03T09:01:32","modified_gmt":"2018-07-03T13:01:32","slug":"genomic-analysis-is-a-snap-using-snapgene","status":"publish","type":"post","link":"https:\/\/info.hsls.pitt.edu\/updatereport\/genomic-analysis-is-a-snap-using-snapgene\/","title":{"rendered":"Genomic Analysis is a Snap using SnapGene"},"content":{"rendered":"<figure style=\"width: 245px\" class=\"wp-caption alignright\"><a href=\"https:\/\/www.snapgene.com\/products\/screenshots\/images\/win\/fullRes\/mapView.png\"><img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/www.snapgene.com\/products\/screenshots\/images\/win\/fullRes\/mapView.png\" alt=\"SnapGene MapView\" width=\"245\" height=\"190\" \/><\/a><figcaption class=\"wp-caption-text\">Image: Restriction Cloning (<a href=\"https:\/\/www.snapgene.com\/products\/screenshots\/\">www.snapgene.com\/products\/screenshots<\/a>) by SnapGene<\/figcaption><\/figure>\n<p>Would you like to\u2026<\/p>\n<ul>\n<li>easily plan and simulate your DNA manipulations?<\/li>\n<li>visualize ORFs, reading frames, and primer binding sites?<\/li>\n<li>automatically record the steps in a cloning project?<\/li>\n<li>share annotated sequence files with other researchers?<\/li>\n<\/ul>\n<p>You can do all of this and more with <a href=\"https:\/\/www.snapgene.com\/\">SnapGene<\/a>. The HSLS Molecular Biology Information Service now provides access to this popular <a href=\"http:\/\/hsls.libguides.com\/molbio\/snapgene\">molecular biology software<\/a>, making it that much easier for Pitt researchers to easily perform <em>in silico<\/em> DNA analysis, molecular cloning, and PCR.<!--more--><\/p>\n<p>Here are just a few of the things SnapGene can do:<\/p>\n<ul>\n<li><strong>In-Fusion Cloning<\/strong>: SnapGene is the first software to simulate Clontech\u2019s versatile method for creating seamless gene fusions. Just select the DNA fragments to fuse, and SnapGene will design the primers.<\/li>\n<li><strong>Gibson Assembly<\/strong>: This technique joins PCR amplified DNA segments to create overlapping ends and inserts fragments into a plasmid without the use of restriction enzymes. SnapGene simplifies the process by automating primer design.<\/li>\n<li><strong>Automatic Documentation: <\/strong>Each procedure is automatically logged in a graphical history and ready to use as an experimental protocol. All ancestor constructs are embedded in the final file, and can be resurrected separately.<\/li>\n<li><strong>Agarose Gel Electrophoresis:<\/strong> Restriction fragments are displayed in three formats: simulated gel, numerical list, and sequence map.<\/li>\n<li><strong>Primers:<\/strong> Revolutionary tools using rigorous thermodynamic algorithms calculate melting temperatures and duplex alignments.<\/li>\n<li><strong>Large Sequences:<\/strong> Large sequences with thousands of annotated features, including chromosome-sized, are browsable thanks to a proprietary MICA algorithm.<\/li>\n<li><strong>Versatile Data Import\/Export:<\/strong> Supported formats include ApE, <a href=\"http:\/\/hsls.libguides.com\/molbio\/lasergene\">Lasergene<\/a>, Gene Construction Kit, GenBank, MacVector, <a href=\"http:\/\/hsls.libguides.com\/molbio\/vnti\">VNTI<\/a>, and more.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.hsls.pitt.edu\/molbio\/software_registration\">Register<\/a> for SnapGene and check out the <a href=\"https:\/\/www.snapgene.com\/support\/tutorial_videos\/introduction_to_snapgene\/\">tutorial videos<\/a>. The <a href=\"https:\/\/www.snapgene.com\/support\/user_guide\">user guide<\/a> offers extensive lessons ranging from searching and colors, to enzymes and restriction cloning &amp; linear ligation. The <a href=\"https:\/\/www.snapgene.com\/support\/frequently_asked_questions\/\">FAQs<\/a> also provide support related to files, features, primers, and more.\u00a0<a href=\"https:\/\/www.hsls.pitt.edu\/ask-a-molbio-specialist\">Contact<\/a> the HSLS <a href=\"https:\/\/www.hsls.pitt.edu\/molbio\">Molecular Biology Information Service<\/a> with any additional questions.<\/p>\n<p>*Parts of this article were derived from the <a href=\"https:\/\/www.snapgene.com\/\">SnapGene website<\/a>.<\/p>\n<p>~ Carrie Iwema<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Would you like to\u2026 easily plan and simulate your DNA manipulations? visualize ORFs, reading frames, and primer binding sites? automatically record the steps in a cloning project? share annotated sequence files with other researchers? You can do all of this and more with SnapGene. The HSLS Molecular Biology Information Service now provides access to this [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"issue-archives","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[125],"tags":[-1],"class_list":["post-11099","post","type-post","status-publish","format-standard","hentry","category-july-2018","avhec_catgroup-issue-archives"],"_links":{"self":[{"href":"https:\/\/info.hsls.pitt.edu\/updatereport\/wp-json\/wp\/v2\/posts\/11099","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/info.hsls.pitt.edu\/updatereport\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/info.hsls.pitt.edu\/updatereport\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/updatereport\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/updatereport\/wp-json\/wp\/v2\/comments?post=11099"}],"version-history":[{"count":8,"href":"https:\/\/info.hsls.pitt.edu\/updatereport\/wp-json\/wp\/v2\/posts\/11099\/revisions"}],"predecessor-version":[{"id":11145,"href":"https:\/\/info.hsls.pitt.edu\/updatereport\/wp-json\/wp\/v2\/posts\/11099\/revisions\/11145"}],"wp:attachment":[{"href":"https:\/\/info.hsls.pitt.edu\/updatereport\/wp-json\/wp\/v2\/media?parent=11099"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/updatereport\/wp-json\/wp\/v2\/categories?post=11099"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/updatereport\/wp-json\/wp\/v2\/tags?post=11099"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}