The HSLS Molecular Biology Information Service (HSLS-MBIS) provides access to many bioinformatics analysis tools, including Partek Flow. University of Pittsburgh researchers are now able to perform chromatin immunoprecipitation sequencing (ChIP-Seq) analysis using Partek software to determine how proteins interact with DNA and regulate gene expression.
ChIP-Seq uses high-throughput DNA sequencing to map protein-DNA interactions across the entire genome. The point-and-click user interface in Partek software helps researchers to quickly build a start-to-finish ChIP-Seq pipeline and:
- identify enriched genomic regions
- filter and annotate genomic regions
- detect known motifs
- discover novel motifs
- visualize enriched genomic regions and motifs
- find enriched gene sets
- integrate with gene expression data
Partek software allows for the easy integration of ChIP-Seq and RNA-Seq studies into a single study. First, peaks and transcription factor binding sites are identified in the ChIP-Seq data, then the peaks are annotated to the genome to locate the nearest transcription start site. Next, a targeted gene list generated from the ChIP-Seq data is compared to a list of differentially expressed genes from RNA-Seq data, followed by visualization using a Venn diagram. The Partek chromosome view allows for the combined visualization of peaks and differentially expressed genes. A webinar describing this process is available.
*Parts of this article content were derived from the Partek ChIP-Seq website.