{"id":5369,"date":"2024-06-20T15:11:53","date_gmt":"2024-06-20T20:11:53","guid":{"rendered":"https:\/\/info.hsls.pitt.edu\/molbio\/?p=5369"},"modified":"2024-06-27T07:48:44","modified_gmt":"2024-06-27T12:48:44","slug":"learn-hsls-single-cell-rna-seq-data-analysis-using-partek-flow","status":"publish","type":"post","link":"https:\/\/info.hsls.pitt.edu\/molbio\/2024\/06\/20\/learn-hsls-single-cell-rna-seq-data-analysis-using-partek-flow\/","title":{"rendered":"Learn @ HSLS: Single Cell RNA-Seq Data Analysis using Partek Flow, June 26"},"content":{"rendered":"<div class=\"field field--name-body field--type-text-with-summary field--label-hidden field__item\">\n<p>Wednesday, June 26, 2024, 1-4 p.m.<br \/>\nOnline<br \/>\n<a href=\"https:\/\/www.hsls.pitt.edu\/instruction\/single-cell-rna-seq-data-analysis-using-partek-flow\/6503\">Register for Single Cell RNA-Seq Data Analysis using Partek Flow<\/a><\/p>\n<p>This workshop is on single cell RNASeq analysis with multiple samples\u00a0using HSLS-licensed\u00a0<a href=\"https:\/\/hsls.libguides.com\/MBIStools\/partekflow\">Partek Flow\u00a0<\/a>software. The workshop is for experimental biologists seeking to learn how to analyze the single cell data generated through experiments or retrieved from a publication or a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user interface, so neither computer programming experience nor familiarity with the command-line interface is required.<\/p>\n<\/div>\n<p><!--more--><\/p>\n<div class=\"field field--name-field-learning-objective field--type-string field--label-above\">\n<div class=\"field__label\"><strong>Upon completing this class, you should be able to:<\/strong><\/div>\n<ul class=\"field field--name-field-learning-objective field--type-string field--label-above field__items\">\n<li class=\"field__item\">analyze from Raw data (FASTQ files)<\/li>\n<li class=\"field__item\">import FASTQ files and run Cellranger software to generate single cell count matrix<\/li>\n<li class=\"field__item\">analyze from Count Matrix data<\/li>\n<li class=\"field__item\">import single cell count matrix<\/li>\n<li class=\"field__item\">perform quality checks (QC) on single-cell data<\/li>\n<li class=\"field__item\">filter and normalize the data<\/li>\n<li class=\"field__item\">generate PCA and t-SNE, UMAP plots to visualize data<\/li>\n<li class=\"field__item\">identify cell populations, and biomarkers in the multisample data<\/li>\n<li class=\"field__item\">compute differential gene expressions between two cell populations\u202fand\/or samples<\/li>\n<\/ul>\n<\/div>\n<div class=\"field field--name-field-class-level field--type-list-string field--label-inline\">\n<div class=\"field__label\"><strong>Level:<\/strong> Novice<\/div>\n<div><\/div>\n<\/div>\n<div class=\"field field--name-field-pre-work-description field--type-text-long field--label-inline\">\n<div class=\"field__label\"><strong>What should you do before attending this class:<\/strong> Register for <a href=\"https:\/\/hsls.libguides.com\/MBISsoftware\/partekflow\">Partek Flow<\/a>\u00a0software.<\/div>\n<div><\/div>\n<\/div>\n<div class=\"field field--name-field-recording-status field--type-list-string field--label-inline\">\n<div class=\"field__label\"><strong>Recording status:<\/strong> This class will be recorded and shared with attendees.<\/div>\n<\/div>\n<div class=\"field field--name-field-class-materials field--type-list-string field--label-inline\">\n<div><\/div>\n<div class=\"field__label\"><strong>Class Materials:<\/strong> Class materials will be shared with attendees.<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Wednesday, June 26, 2024, 1-4 p.m.<br \/>\nOnline<br \/>\n<a href=\"https:\/\/www.hsls.pitt.edu\/instruction\/single-cell-rna-seq-data-analysis-using-partek-flow\/6503\">Register for Single Cell RNA-Seq Data Analysis using Partek Flow<\/a><\/p>\n<p>This workshop is on single cell RNASeq analysis with multiple samples\u00a0using HSLS-licensed\u00a0<a href=\"https:\/\/hsls.libguides.com\/MBIStools\/partekflow\">Partek Flow\u00a0<\/a>software.<\/p>\n<p><a class=\"read-more\" alt= \"Learn @ HSLS: Single Cell RNA-Seq Data Analysis using Partek Flow, June 26\" href=\"https:\/\/info.hsls.pitt.edu\/molbio\/2024\/06\/20\/learn-hsls-single-cell-rna-seq-data-analysis-using-partek-flow\/\">Read more&hellip;<\/a><\/p>\n","protected":false},"author":46,"featured_media":5378,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[45],"tags":[],"class_list":["post-5369","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-june-2024"],"_links":{"self":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/5369","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/users\/46"}],"replies":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/comments?post=5369"}],"version-history":[{"count":4,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/5369\/revisions"}],"predecessor-version":[{"id":5373,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/5369\/revisions\/5373"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/media\/5378"}],"wp:attachment":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/media?parent=5369"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/categories?post=5369"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/tags?post=5369"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}