{"id":5258,"date":"2024-02-01T13:47:15","date_gmt":"2024-02-01T18:47:15","guid":{"rendered":"https:\/\/info.hsls.pitt.edu\/molbio\/?p=5258"},"modified":"2024-02-15T15:22:04","modified_gmt":"2024-02-15T20:22:04","slug":"february-7-single-cell-rna-seq-data-analysis-using-partek-flow","status":"publish","type":"post","link":"https:\/\/info.hsls.pitt.edu\/molbio\/2024\/02\/01\/february-7-single-cell-rna-seq-data-analysis-using-partek-flow\/","title":{"rendered":"February 7: Single Cell RNA-Seq Data Analysis using Partek Flow"},"content":{"rendered":"<p>Wednesday, February 7, 2024, 1-4 p.m.<br \/>\nHybrid session: Attend online or in person in the Falk Library Classroom<br \/>\n<a href=\"https:\/\/www.hsls.pitt.edu\/instruction\/single-cell-rna-seq-data-analysis-using-partek-flow\/6503\">Register for Single Cell RNA-Seq Data Analysis using Partek Flow<\/a><\/p>\n<p>This workshop is on single cell RNASeq analysis with multiple samples\u00a0using HSLS-licensed\u00a0<a href=\"https:\/\/hsls.libguides.com\/MBIStools\/partekflow\">Partek Flow\u00a0<\/a>software.<\/p>\n<p>Target Audience:<\/p>\n<p>Experimental biologists seeking to learn how to analyze the single cell data generated through experiments or retrieved from a publication or a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user interface, so neither computer programming experience nor familiarity with the command-line interface is required.<\/p>\n<p><!--more--><\/p>\n<p>Upon completing this class, you should be able to:<\/p>\n<ul>\n<li>\u00a0analyze from Raw data (FASTQ files)<\/li>\n<li>import FASTQ files and run Cellranger software to generate single cell count matrix<\/li>\n<li>analyze from Count Matrix data<\/li>\n<li>import single cell count matrix<\/li>\n<li>perform quality checks (QC) on single-cell data<\/li>\n<li>filter and normalize the data<\/li>\n<li>generate PCA and t-SNE, UMAP plots to visualize data<\/li>\n<li>identify cell populations, and biomarkers in the multisample data<\/li>\n<li>compute differential gene expressions between two cell populations\u202fand\/or samples<\/li>\n<\/ul>\n<p>Level: novice<\/p>\n<p>This class is recorded and sent to attendees.<\/p>\n<p>Class materials will be shared with attendees.<\/p>\n<p>What should you do to prepare before attending this class: Register for\u00a0<a href=\"https:\/\/hsls.libguides.com\/MBISsoftware\/partekflow\">Partek Flow<\/a>\u00a0software.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Wednesday, February 7, 2024, 1-4 p.m.<br \/>\nHybrid session: Attend online or in person in the Falk Library Classroom<br \/>\n<a href=\"https:\/\/www.hsls.pitt.edu\/instruction\/single-cell-rna-seq-data-analysis-using-partek-flow\/6503\">Register for Single Cell RNA-Seq Data Analysis using Partek Flow<\/a><\/p>\n<p>This workshop is on single cell RNASeq analysis with multiple samples\u00a0using HSLS-licensed\u00a0<a href=\"https:\/\/hsls.libguides.com\/MBIStools\/partekflow\">Partek Flow\u00a0<\/a>software.<\/p>\n<p><a class=\"read-more\" alt= \"February 7: Single Cell RNA-Seq Data Analysis using Partek Flow\" href=\"https:\/\/info.hsls.pitt.edu\/molbio\/2024\/02\/01\/february-7-single-cell-rna-seq-data-analysis-using-partek-flow\/\">Read more&hellip;<\/a><\/p>\n","protected":false},"author":46,"featured_media":5170,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[40],"tags":[],"class_list":["post-5258","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-february-2024"],"_links":{"self":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/5258","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/users\/46"}],"replies":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/comments?post=5258"}],"version-history":[{"count":1,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/5258\/revisions"}],"predecessor-version":[{"id":5259,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/5258\/revisions\/5259"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/media\/5170"}],"wp:attachment":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/media?parent=5258"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/categories?post=5258"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/tags?post=5258"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}