{"id":5075,"date":"2023-05-30T14:39:55","date_gmt":"2023-05-30T19:39:55","guid":{"rendered":"https:\/\/info.hsls.pitt.edu\/molbio\/?p=5075"},"modified":"2023-06-07T11:13:43","modified_gmt":"2023-06-07T16:13:43","slug":"may-31-scrna-seq-data-analysis-using-partekflow","status":"publish","type":"post","link":"https:\/\/info.hsls.pitt.edu\/molbio\/2023\/05\/30\/may-31-scrna-seq-data-analysis-using-partekflow\/","title":{"rendered":"May 31: scRNA-Seq Data Analysis using PartekFlow"},"content":{"rendered":"<p>Wednesday, May 31, 2023, 1-3 p.m., online<\/p>\n<p><a href=\"https:\/\/www.hsls.pitt.edu\/instruction\/scrna-seq-data-analysis-using-partekflow\/6503\">Register for scRNA-Seq Data Analysis using PartekFlow<\/a><\/p>\n<p>In this class, you will learn how to analyze scRNA-Seq data using the user-friendly Partek Flow software. The class will cover a range of topics, from data import to cell population identification and differential gene expression analysis.<\/p>\n<p><!--more--><\/p>\n<p><strong>Learning Objectives<\/strong>:<\/p>\n<p>Upon completing this class, you should be able to:<\/p>\n<ul>\n<li>Import FASTQ\/Count matrix files from an scRNA-Seq experiment into Partek Flow<\/li>\n<li>Run Cell Ranger software on FASTQ files to generate count matrix<\/li>\n<li>Perform quality checks (QC) on single-cell data<\/li>\n<li>Filter and normalize the data<\/li>\n<li>Generate PCA, t-SNE, and UMAP plots to visualize data<\/li>\n<li>Identify cell populations, biomarkers, and compute differential gene expressions between two cell populations<\/li>\n<\/ul>\n<p>Target Audience:<\/p>\n<p>Experimental biologists seeking to analyze scRNA-Seq data generated through experiments or retrieved from a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user interface, so neither programming experience nor familiarity with the command line interface is required.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Wednesday, May 31, 2023, 1-3 p.m., online<\/p>\n<p><a href=\"https:\/\/www.hsls.pitt.edu\/instruction\/scrna-seq-data-analysis-using-partekflow\/6503\">Register for scRNA-Seq Data Analysis using PartekFlow<\/a><\/p>\n<p>In this class, you will learn how to analyze scRNA-Seq data using the user-friendly Partek Flow software. The class will cover a range of topics, from data import to cell population identification and differential gene expression analysis.<\/p>\n<p><a class=\"read-more\" alt= \"May 31: scRNA-Seq Data Analysis using PartekFlow\" href=\"https:\/\/info.hsls.pitt.edu\/molbio\/2023\/05\/30\/may-31-scrna-seq-data-analysis-using-partekflow\/\">Read more&hellip;<\/a><\/p>\n","protected":false},"author":46,"featured_media":4875,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[28],"tags":[],"class_list":["post-5075","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-may-2023"],"_links":{"self":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/5075","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/users\/46"}],"replies":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/comments?post=5075"}],"version-history":[{"count":3,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/5075\/revisions"}],"predecessor-version":[{"id":5092,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/5075\/revisions\/5092"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/media\/4875"}],"wp:attachment":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/media?parent=5075"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/categories?post=5075"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/tags?post=5075"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}