This information is over 2 years old. Information was current at time of publication.{"id":4781,"date":"2022-05-23T10:25:29","date_gmt":"2022-05-23T15:25:29","guid":{"rendered":"https:\/\/info.hsls.pitt.edu\/molbio\/?p=4781"},"modified":"2022-06-30T10:19:30","modified_gmt":"2022-06-30T15:19:30","slug":"june-29-scrna-seq-data-analysis-using-partekflow","status":"publish","type":"post","link":"https:\/\/info.hsls.pitt.edu\/molbio\/2022\/05\/23\/june-29-scrna-seq-data-analysis-using-partekflow\/","title":{"rendered":"June 29- scRNA-Seq Data Analysis Using PartekFlow"},"content":{"rendered":"<p><a href=\"https:\/\/www.hsls.pitt.edu\/instruction\/scrna-seq-data-analysis-using-partekflow-person\/7846\">Register for scRNA-Seq Data Analysis Using PartekFlow<\/a><\/p>\n<p>June 29 11 a.m. &#8211; 1 p.m., in Falk Library Classroom 2<\/p>\n<p>This is\u00a0a<strong>\u00a0flipped class;<\/strong>\u00a0links to\u00a0PowerPoint slides,\u00a0<strong>lecture videos<\/strong>, and\u00a0practice exercises\u00a0that you can view on your own schedule are available upon registration. During this in-person hands-on session, you will learn how to solve the exercise problems.<\/p>\n<p>The workshop lecture videos will introduce the techniques, platforms, and methods used in generating single-cell RNA-Seq data, followed by a step-by-step guide on scRNA-seq data analysis using HSLS-licensed\u00a0<a href=\"http:\/\/hsls.libguides.com\/molbio\/partekflow\">Partek Flow<\/a>\u00a0software.<!--more--><\/p>\n<p>Participants will learn how to<\/p>\n<ul>\n<li>import FASTQ\/Count matrix files from scRNA-Seq experiments to PartekFlow software<\/li>\n<li>perform quality checks (QC) on single-cell data<\/li>\n<li>filter and normalize the data<\/li>\n<li>generate PCA and t-SNE plots to visualize data<\/li>\n<li>identify cell populations, biomarkers, and compute differential gene expressions between two cell populations<\/li>\n<li>perform trajectory analysis<\/li>\n<\/ul>\n<p>Target Audience:<\/p>\n<p>Experimental biologists seeking to analyze scRNA-Seq data generated through experiments or retrieved from a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user interface, so neither programming experience nor familiarity with the command line interface is required.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p><a href=\"https:\/\/www.hsls.pitt.edu\/instruction\/scrna-seq-data-analysis-using-partekflow-person\/7846\">Register for scRNA-Seq Data Analysis Using PartekFlow<\/a><\/p>\n<p>June 29 11 a.m. &#8211; 1 p.m., in Falk Library Classroom 2<\/p>\n<p>This is\u00a0a<strong>\u00a0flipped class;<\/strong>\u00a0links to\u00a0PowerPoint slides,\u00a0<strong>lecture videos<\/strong>, and\u00a0practice exercises\u00a0that you can view on your own schedule are available upon registration.<\/p>\n<p><a class=\"read-more\" alt= \"June 29- scRNA-Seq Data Analysis Using PartekFlow\" href=\"https:\/\/info.hsls.pitt.edu\/molbio\/2022\/05\/23\/june-29-scrna-seq-data-analysis-using-partekflow\/\">Read more&hellip;<\/a><\/p>\n","protected":false},"author":38,"featured_media":4784,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[1],"tags":[],"class_list":["post-4781","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/4781","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/users\/38"}],"replies":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/comments?post=4781"}],"version-history":[{"count":4,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/4781\/revisions"}],"predecessor-version":[{"id":4788,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/4781\/revisions\/4788"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/media\/4784"}],"wp:attachment":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/media?parent=4781"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/categories?post=4781"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/tags?post=4781"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}