This information is over 2 years old. Information was current at time of publication.{"id":2471,"date":"2018-01-02T11:27:41","date_gmt":"2018-01-02T16:27:41","guid":{"rendered":"http:\/\/info.hsls.pitt.edu\/molbio\/?p=2471"},"modified":"2018-01-02T11:27:41","modified_gmt":"2018-01-02T16:27:41","slug":"110-wksp-rna-seq","status":"publish","type":"post","link":"https:\/\/info.hsls.pitt.edu\/molbio\/2018\/01\/02\/110-wksp-rna-seq\/","title":{"rendered":"1\/10 wksp: RNA-Seq"},"content":{"rendered":"<p><strong><span style=\"color: navy\">You MUST register for ALL of the following:<\/li>\n<p><\/strong><\/p>\n<ul>\n<li><a href=\"https:\/\/www.hsls.pitt.edu\/instruction\/molbio\/rna-seq-clc-genomics\/354\">TO ATTEND THE WORKSHOP<\/a><\/li>\n<li><a href=\"https:\/\/crc.pitt.edu\/apply\/\">Center for Research Computing HPC allocation<\/a> <\/li>\n<li><a href=\"http:\/\/hsls.libguides.com\/molbio\/clcgenomics\">CLC Genomics Workbench<\/a> <\/li>\n<li><a href=\"http:\/\/hsls.libguides.com\/molbio\/biomedgen\">Biomedical Genomics Workbench<\/a> <\/li>\n<\/ul>\n<li>\n<strong><span style=\"color: deepskyblue\">WHEN:<\/span> <\/strong>January 10th, 1-4 pm<\/li>\n<li><strong><span style=\"color: deepskyblue\">WHERE:<\/span> <\/strong>Falk Library, Classroom 2<\/li>\n<li><strong><span style=\"color: deepskyblue\">WHO:<\/span> <\/strong>Ansuman Chattopadhyay, PhD<\/li>\n<p>Massively parallel RNA sequencing, commonly known as RNA-Seq, provides a more detailed picture of transcriptomes compared to microarray profiling. As Next-Generation Sequencing technologies are becoming faster and less expensive, RNA-Seq is now the primary assay of choice for researchers investigating the abundance and diversity of RNA transcripts. Analysis of the large-scale data sets generated by a typical RNA-Seq experiment is challenging as it demands access to powerful computers and researcher training to run sophisticated bioinformatics software packages. <\/p>\n<p>This hands-on workshop is an introduction to RNA-Seq analysis using the library-licensed CLC Genomics Workbench. Participants will learn how to:<\/p>\n<ul>\n<li>import reads from an NCBI GEO dataset (GSE52778)<\/li>\n<li>align RNA-Seq data to a reference genome<\/li>\n<li>calculate known genes and transcript expressions<\/li>\n<li>perform differential expression analysis<\/li>\n<\/ul>\n<p><a href=\"http:\/\/files.hsls.pitt.edu\/files\/molbio\/MolbioWorkshops.pdf\">HSLS Winter\/Spring MolBio Info Service Training Workshops schedule<\/a><\/p>\n<p>Please <a href=\"https:\/\/www.hsls.pitt.edu\/ask-a-molbio-specialist\">contact<\/a> the <a href=\"http:\/\/www.hsls.pitt.edu\/molbio\">HSLS Molecular Biology Information Service<\/a> with any questions.<\/p>\n","protected":false},"excerpt":{"rendered":"<p><strong><span style=\"color: navy\">You MUST register for ALL of the following:<\/p>\n<p><\/span><\/strong><\/p>\n<ul>\n<li><a href=\"https:\/\/www.hsls.pitt.edu\/instruction\/molbio\/rna-seq-clc-genomics\/354\">TO ATTEND THE WORKSHOP<\/a><\/li>\n<li><a href=\"https:\/\/crc.pitt.edu\/apply\/\">Center for Research Computing HPC allocation<\/a> <\/li>\n<li><a href=\"http:\/\/hsls.libguides.com\/molbio\/clcgenomics\">CLC Genomics Workbench<\/a> <\/li>\n<li><a href=\"http:\/\/hsls.libguides.com\/molbio\/biomedgen\">Biomedical Genomics Workbench<\/a> <\/li>\n<\/ul>\n<li>\n<strong><span style=\"color: deepskyblue\">WHEN:<\/span> <\/strong>January 10th, 1-4 pm<\/li>\n<li><strong><span style=\"color: deepskyblue\">WHERE:<\/span> <\/strong>Falk Library, Classroom 2<\/li>\n<li><strong><span style=\"color: deepskyblue\">WHO:<\/span> <\/strong>Ansuman Chattopadhyay, PhD<\/li>\n<p>Massively parallel RNA sequencing, commonly known as RNA-Seq, provides a more detailed picture of transcriptomes compared to microarray profiling.<\/p>\n<p><a class=\"read-more\" alt= \"1\/10 wksp: RNA-Seq\" href=\"https:\/\/info.hsls.pitt.edu\/molbio\/2018\/01\/02\/110-wksp-rna-seq\/\">Read more&hellip;<\/a><\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[13],"tags":[],"class_list":["post-2471","post","type-post","status-publish","format-standard","hentry","category-workshops"],"_links":{"self":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/2471","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/comments?post=2471"}],"version-history":[{"count":9,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/2471\/revisions"}],"predecessor-version":[{"id":2480,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/2471\/revisions\/2480"}],"wp:attachment":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/media?parent=2471"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/categories?post=2471"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/tags?post=2471"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}