This information is over 2 years old. Information was current at time of publication.{"id":2060,"date":"2016-09-07T10:27:01","date_gmt":"2016-09-07T15:27:01","guid":{"rendered":"http:\/\/info.hsls.pitt.edu\/molbio\/?p=2060"},"modified":"2016-09-07T10:27:01","modified_gmt":"2016-09-07T15:27:01","slug":"rna-seq-wksp-on-914","status":"publish","type":"post","link":"https:\/\/info.hsls.pitt.edu\/molbio\/2016\/09\/07\/rna-seq-wksp-on-914\/","title":{"rendered":"RNA-seq wksp on 9\/14"},"content":{"rendered":"<p><strong><span style=\"color: blue\">You MUST register for ALL of the following:<\/li>\n<p><\/strong><\/p>\n<ul>\n<li><a href=\"http:\/\/www.hsls.pitt.edu\/class\/desc\/molecular-biology\/rna-seq-analysis-clc-genomics-workbench\/1304\">TO ATTEND THE WORKSHOP<\/a><\/li>\n<li><a href=\"http:\/\/core.sam.pitt.edu\/apply\">FRANK\/SAM Account<\/a> <\/li>\n<li><a href=\"http:\/\/hsls.libguides.com\/molbio\/clcgenomics\">CLCbio Genomics Workbench<\/a> <\/li>\n<\/ul>\n<li>\n<strong><span style=\"color: skyblue\">WHEN:<\/span> <\/strong>September 14th, 1-4 pm<\/li>\n<li><strong><span style=\"color: skyblue\">WHERE:<\/span> <\/strong>Falk Library, Classroom 2<\/li>\n<li><strong><span style=\"color: skyblue\">WHO:<\/span> <\/strong>Ansuman Chattopadhyay, PhD<\/li>\n<p>Massively parallel RNA sequencing, commonly known as RNA-seq, provides a more detailed picture of transcriptomes compared to microarray profiling. As Next-Generation Sequencing technologies are becoming faster and less expensive, RNA-seq is now the primary assay of choice for researchers investigating the abundance and diversity of RNA transcripts. Analysis of the large-scale data sets generated by a typical RNA-seq experiment is challenging as it demands access to powerful computers and researcher training to run sophisticated bioinformatics software packages. This workshop will teach how to analyze sample RNA-seq data using <a href=\"http:\/\/hsls.libguides.com\/molbio\/clcgenomics\">CLCbio Genomics Workbench<\/a> software and the CLCbio Genomics Server installed at the <a href=\"http:\/\/core.sam.pitt.edu\/node\/85\">Pitt FRANK Cluster<\/a>. <\/p>\n<p>Participants will gain hands-on experience to:<\/p>\n<ul>\n<li>Download biological sequence data from NCBI Sequence Read Archive (SRA)\/European Nucleotide Archive (ENA)<\/li>\n<li>Align reads to the human reference genome<\/li>\n<li>Estimate expression of known genes and transcripts<\/li>\n<li>Perform differential expression analysis at the gene and transcript isoform level<\/li>\n<\/ul>\n<p>Please <a href=\"http:\/\/www.hsls.pitt.edu\/molbio\/askmolbio\">contact<\/a> the <a href=\"http:\/\/www.hsls.pitt.edu\/molbio\">HSLS Molecular Biology Information Service<\/a> with any questions.<\/p>\n","protected":false},"excerpt":{"rendered":"<p><strong><span style=\"color: blue\">You MUST register for ALL of the following:<\/p>\n<p><\/span><\/strong><\/p>\n<ul>\n<li><a href=\"http:\/\/www.hsls.pitt.edu\/class\/desc\/molecular-biology\/rna-seq-analysis-clc-genomics-workbench\/1304\">TO ATTEND THE WORKSHOP<\/a><\/li>\n<li><a href=\"http:\/\/core.sam.pitt.edu\/apply\">FRANK\/SAM Account<\/a> <\/li>\n<li><a href=\"http:\/\/hsls.libguides.com\/molbio\/clcgenomics\">CLCbio Genomics Workbench<\/a> <\/li>\n<\/ul>\n<li>\n<strong><span style=\"color: skyblue\">WHEN:<\/span> <\/strong>September 14th, 1-4 pm<\/li>\n<li><strong><span style=\"color: skyblue\">WHERE:<\/span> <\/strong>Falk Library, Classroom 2<\/li>\n<li><strong><span style=\"color: skyblue\">WHO:<\/span> <\/strong>Ansuman Chattopadhyay, PhD<\/li>\n<p>Massively parallel RNA sequencing, commonly known as RNA-seq, provides a more detailed picture of transcriptomes compared to microarray profiling.<\/p>\n<p><a class=\"read-more\" alt= \"RNA-seq wksp on 9\/14\" href=\"https:\/\/info.hsls.pitt.edu\/molbio\/2016\/09\/07\/rna-seq-wksp-on-914\/\">Read more&hellip;<\/a><\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[13],"tags":[],"class_list":["post-2060","post","type-post","status-publish","format-standard","hentry","category-workshops"],"_links":{"self":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/2060","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/comments?post=2060"}],"version-history":[{"count":2,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/2060\/revisions"}],"predecessor-version":[{"id":2068,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/2060\/revisions\/2068"}],"wp:attachment":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/media?parent=2060"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/categories?post=2060"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/tags?post=2060"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}