This information is over 2 years old. Information was current at time of publication.{"id":1140,"date":"2014-10-27T12:19:53","date_gmt":"2014-10-27T17:19:53","guid":{"rendered":"http:\/\/info.hsls.pitt.edu\/molbio\/?p=1140"},"modified":"2014-10-27T12:19:53","modified_gmt":"2014-10-27T17:19:53","slug":"how-to-talk-elispot-immune-responses","status":"publish","type":"post","link":"https:\/\/info.hsls.pitt.edu\/molbio\/2014\/10\/27\/how-to-talk-elispot-immune-responses\/","title":{"rendered":"How-To Talk: ELISPOT &amp; Immune Responses"},"content":{"rendered":"<p><strong><span style=\"color: blue;font-size: medium\"><big>How to do an ELISPOT to detect immune responses <em>in vitro<\/em><\/big><\/span><\/strong><br \/>\n<strong><span style=\"font-size: medium\">by Tatiana Garcia-Bates, PhD<br \/>\n<\/span><\/strong><br \/>\n<strong><span style=\"color: orange;font-size: medium\">Tuesday, 4th November 2014<\/p>\n<p>10-11 am<br \/>\nFalk Library Classroom 2 <\/span><\/strong><\/p>\n<p>This talk will include how to detect inflammatory cytokine secretion by T cells  after they are challenged with antigen in vitro. I will also review the use of ELISPOT to detect antibody secretion by plasmablasts in the periphery after antigen stimulation. <\/p>\n<p>Registration is NOT required.<\/p>\n","protected":false},"excerpt":{"rendered":"<p><strong><span style=\"color: blue;font-size: medium\">How to do an ELISPOT to detect immune responses <em>in vitro<\/em><\/span><\/strong><br \/>\n<strong><span style=\"font-size: medium\">by Tatiana Garcia-Bates, PhD<br \/>\n<\/span><\/strong><br \/>\n<strong><span style=\"color: orange;font-size: medium\">Tuesday, 4th November 2014<\/span><\/strong><\/p>\n<p>10-11 am<br \/>\nFalk Library Classroom 2 <\/p>\n<p>This talk will include how to detect inflammatory cytokine secretion by T cells  after they are challenged with antigen in vitro.<\/p>\n<p><a class=\"read-more\" alt= \"How-To Talk: ELISPOT &amp; Immune Responses\" href=\"https:\/\/info.hsls.pitt.edu\/molbio\/2014\/10\/27\/how-to-talk-elispot-immune-responses\/\">Read more&hellip;<\/a><\/p>\n","protected":false},"author":31,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[14],"tags":[],"class_list":["post-1140","post","type-post","status-publish","format-standard","hentry","category-interesting"],"_links":{"self":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/1140","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/users\/31"}],"replies":[{"embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/comments?post=1140"}],"version-history":[{"count":5,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/1140\/revisions"}],"predecessor-version":[{"id":1151,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/posts\/1140\/revisions\/1151"}],"wp:attachment":[{"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/media?parent=1140"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/categories?post=1140"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/info.hsls.pitt.edu\/molbio\/wp-json\/wp\/v2\/tags?post=1140"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}