- WHAT: ChIP-Seq & Partek Flow
- WHEN: October 23rd, 1-4pm
- WHERE: Scaife Hall, Falk Library, Classroom 2
- WHO: Ansuman Chattopahyay & Sri Chaparala
- HOW: Register here
Workshop Objective:
This 3-hour hands-on workshop provides a brief introduction to techniques, platforms, and methods used in chromatin profiling experiments, including Transcription Factor / Histone ChIP-Seq and ATAC-Seq. We will focus on data analysis using HSLS-licensed Partek Flow software.
Participants will learn how to
- import raw data from a GEO dataset
- align ChIP-Seq reads to a reference genome
- identify and annotate peaks
- visualize and summarize the results
- search for known transcription factor binding motifs
- integrate ChIP-Seq results with bulk RNA-Seq data
Target Audience:
Experimental biologists seeking to analyze ChIP-Seq data generated through experiments or retrieved from a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user Interface, so neither programming experience nor familiarity with command line interface is required.
Workshop Requirements:
Workshop Guide:
Suggested Reading:
Attribution:
Please include the following statement in the acknowledgments section for all publications, posters, and presentations: Data analysis was performed using {name of software} software licensed through the Molecular Biology Information Service of the Health Sciences Library System, University of Pittsburgh.
HSLS Fall 2019 MolBio Info Service Training Workshops schedule
Contact the HSLS Molecular Biology Information Service with any questions.