You MUST register for ALL of the following:
- TO ATTEND THE WORKSHOP
- Center for Research Computing HPC allocation. Under “description” write CLC Genomics.
- CLC Genomics Workbench
- Biomedical Genomics Workbench
- WHEN: April 25th, 1-4 pm
- WHERE: Falk Library, Classroom 2
- WHO: Ansuman Chattopadhyay, PhD
Massively parallel RNA sequencing, commonly known as RNA-Seq, provides a more detailed picture of transcriptomes compared to microarray profiling. As Next-Generation Sequencing technologies are becoming faster and less expensive, RNA-Seq is now the primary assay of choice for researchers investigating the abundance and diversity of RNA transcripts. Analysis of the large-scale data sets generated by a typical RNA-Seq experiment is challenging as it demands access to powerful computers and researcher training to run sophisticated bioinformatics software packages.
This hands-on workshop is an introduction to RNA-Seq analysis using the library-licensed CLC Genomics Workbench. Participants will learn how to:
- import reads from an NCBI GEO dataset (GSE52778)
- align RNA-Seq data to a reference genome
- calculate known genes and transcript expressions
- perform differential expression analysis
HSLS Winter/Spring MolBio Info Service Training Workshops schedule
Please contact the HSLS Molecular Biology Information Service with any questions.