You MUST register for ALL of the following:

  • WHEN: March 28th, 1-4 pm
  • WHERE: Falk Library, Classroom 2
  • WHO: Ansuman Chattopadhyay, PhD
  • Massively parallel RNA sequencing, commonly known as RNA-Seq, provides a more detailed picture of transcriptomes compared to microarray profiling. As Next-Generation Sequencing technologies are becoming faster and less expensive, RNA-Seq is now the primary assay of choice for researchers investigating the abundance and diversity of RNA transcripts. Analysis of the large-scale data sets generated by a typical RNA-Seq experiment is challenging as it demands access to powerful computers and researcher training to run sophisticated bioinformatics software packages.

    This hands-on workshop is an introduction to RNA-Seq analysis using the library-licensed CLC Genomics Workbench. Participants will learn how to:

    • import reads from an NCBI GEO dataset (GSE52778)
    • align RNA-Seq data to a reference genome
    • calculate known genes and transcript expressions
    • perform differential expression analysis

    HSLS Winter/Spring MolBio Info Service Training Workshops schedule

    Please contact the HSLS Molecular Biology Information Service with any questions.