You MUST register for ALL of the following:
Massively parallel RNA sequencing, commonly known as RNA-seq, provides a more detailed picture of transcriptomes compared to microarray profiling. As Next-Generation Sequencing technologies are becoming faster and less expensive, RNA-seq is now the primary assay of choice for researchers investigating the abundance and diversity of RNA transcripts. Analysis of the large-scale data sets generated by a typical RNA-seq experiment is challenging as it demands access to powerful computers and researcher training to run sophisticated bioinformatics software packages. This workshop will teach how to analyze sample RNA-seq data using CLCbio Genomics Workbench software and the CLCbio Genomics Server installed at the Pitt FRANK Cluster.
Participants will gain hands-on experience to:
- Download biological sequence data from NCBI Sequence Read Archive (SRA)/European Nucleotide Archive (ENA)
- Align reads to the human reference genome
- Estimate expression of known genes and transcripts
- Perform differential expression analysis at the gene and transcript isoform level
Please contact the HSLS Molecular Biology Information Service with any questions.